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Just after incorporating streptavidin-coated microparticles, the elaborate is sure to the strong period via conversation with biotin and streptavidin. The response combination is aspirated in to the measuring cell, where the microparticles are magnetically captured onto the area of your electrode. Unbound substances are removed, and a voltage is placed on the electrode.[31]
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This IgG need to neutralize the more routinely encountered HAMA.[ninety] Steps the laboratory will take To guage this probability include things like applying a larger dilution from the sample having a reagent made up of the non-immune mouse IgG or performing the Evaluation over a in a different way configured assay, ideally a person using different species of reagent antibodies.[91]
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